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dawn heleos ii mals instrument  (Waters Corporation)
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Waters Corporation dawn heleos ii mals instrument
(a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) <t>SEC-MALS</t> analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.
Dawn Heleos Ii Mals Instrument, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dawn heleos2 mals instrument  (Waters Corporation)
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Waters Corporation dawn heleos2 mals instrument
(a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) <t>SEC-MALS</t> analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.
Dawn Heleos2 Mals Instrument, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dawn heleos2 mals instrument/product/Waters Corporation
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mals instrument  (Waters Corporation)
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Waters Corporation mals instrument
(a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) <t>SEC-MALS</t> analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.
Mals Instrument, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wyatt minidawn mals instrument  (Waters Corporation)
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Waters Corporation wyatt minidawn mals instrument
(a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) <t>SEC-MALS</t> analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.
Wyatt Minidawn Mals Instrument, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wyatt instruments mals light  (Waters Corporation)
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Waters Corporation wyatt instruments mals light
(a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) <t>SEC-MALS</t> analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.
Wyatt Instruments Mals Light, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dawn heleos2 mals instrument 578  (Waters Corporation)
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Waters Corporation dawn heleos2 mals instrument 578
(a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) <t>SEC-MALS</t> analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.
Dawn Heleos2 Mals Instrument 578, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wyattqels  (Waters Corporation)
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Waters Corporation wyattqels
(a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) <t>SEC-MALS</t> analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.
Wyattqels, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) SEC-MALS analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.

Journal: bioRxiv

Article Title: Arbitrium systems control lysis/lysogeny through the regulation of small antirepressor proteins

doi: 10.1101/2025.11.23.689978

Figure Lengend Snippet: (a) Bacterial two-hybrid results for interaction between AimX and AimC of ϕ106. Marked are the components fused to the T25 and T18 parts of the Cya protein and the respective mean activity for three independent repeats. Activity in each of three days was normalized by the activity level of the positive Leucine Zipper (LZ) standard control of the assay. (b) SEC-MALS analysis of AimC (magenta) and AimC-AimX (blue) for f106 (left) and BMBtp14 (middle). Chromatograms show the readings from the light scattering (light pink for AimC+AimX and light blue for AimC), refractive index (purple for AimC+AimX and blue for AimC), and UV (dark magenta for AimC+AimX and dark blue for AimC). The horizontal curves represent the calculated molecular masses. Experimental and theoretical molecular weights (MV) for AimC dimers (dimer-tetramer in the case of aimC Btp14 ) and AimC-AimX heterocomplex are indicated in kDa. (c) Gel shift experiments (EMSA) after incubation of AimC repressors from ϕ106 and BMBtp14 phages with DNA fragments containing the relatives aimC - aimL intergenic regions and in presence (1:2 AimC:AimX) or absence of the corresponding AimX antirepressor.

Article Snippet: A Wyatt DAWN HELEOS-II MALS instrument and a Wyatt Optilab rEX differential refractometer (Wyatt) coupled to a Shimadzu HPLC were used to perform SEC-MALS experiments.

Techniques: Activity Assay, Control, Refractive Index, Gel Shift, Incubation